RNA Sequencing techniques
This short video gives you a short introduction to the techniques of RNA sequencing:
- Assessing the quality of the input RNA,
- Protocols to prepare a sequencing RNA library (Illumina example),
- Illumina Next Generation “sequencing by synthesis” workflow,
- Output of the sequencing process, quality check of the produced sequences.
differential gene expression analysis
This video addresses the main steps performed after the bioinformatic parts of RNA-seq. It shows the main inputs (gene counts, sample to conditions) and the main outputs (table of differential genes, volcano plot) that one can get from a classical differential expression analysis.
Please refer to the RNA-seq lesson section “Differential expression” for additional info and practical know-how.